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筛选条件 : Yong HUANG
Xiao Zha; Yongrui Yu; Yong Huang
Environmental Technology and Innovation, 2025 37 - EI SCIE

摘要 : An innovative kind of coarse flocculant employs captured organics from wastewater as a raw material was proposed in our previous research, which is subsequently utilized to capture organics from wastewater in return. Coarse flocculants with different molecular weight, zeta potential and charge density were produced by adjusting modification condition. They were characterized through various methods, while the flocculation effect and structure-activity relationship of the coarse flocculant was also evaluated. The flocculation performance is primarily determined by the charge properties. The molecular weight significantly influences the adsorption-bridging and catching-sweeping effect, thereby affecting floc size and stability. The charge density has important impact on the colloidal particle instability, which in turn impacts floc size. The flocculation mechanisms were discussed based on the structure-activity relationship. Notably, when zeta potential ranges from 7 ± 0.02–9.5 ± 0.02 mV and charge density exceeds 1.35 ± 0.01 mmol·L −1, the charge neutralization effect is maximized. Large floc particle helps enhance organic matter capturing. The results help to guide the development of the novel flocculant.

Xi Cao; Tianqi Liu; Xiang Li; Yong Huang; Qin Nie; Ming Li
Water Research X, 2025 26 - EI SCIE

摘要 : A full-scale simultaneous partial nitrification, anaerobic ammonia oxidation (anammox), and denitrification (SNAD) reactor was initiated to address the problem of high energy consumption for the treatment of low C/N wastewater. The SNAD system achieved a nitrogen removal rate of 0.9 kg/(m 3 ·d) at an influent NH4 + –N concentration of 500 mg/L after 450 days of stable operation. Partial nitrification was achieved by maintaining free ammonia levels at 0.8 ± 0.3 mg/L and dissolved oxygen concentrations between 0.3 mg/L and 1.2 mg/L, which resulted in synergistic nitrogen removal, with anammox contributing 61 % and denitrification contributing 39 %. Microbiological analyses indicated that the dominant microorganisms were Candidatus Brocadia, Thauera, Denitratisoma, and Nitrosomonas . In conclusion, study provides a solid foundation for the broader implementation of the SNAD process in wastewater treatment systems.

Jiahong Ye; Xiang Li; Yan Yuan; Yayi Wang; Yong Huang; Jun Ma
Journal of Environmental Sciences, 2025 152 - EI SCIE

摘要 : Red anaerobic ammonia oxidation (Anammox) granular sludge (AnGS) has been reported in successfully operating Anammox systems, and its color is associated with sludge activity. However, in long-term operating systems, AnGS exhibits different sensory colors, physical structures, community structures, and denitrification performance, but the relationship between them has not yet been elucidated. The AnGS of the Anammox system, which has been in operation for more than a decade, can be divided into two main categories: red and white. The specific Anammox activity (SAA) in conventional red AnGS increased continuously as the particle size increased from <0.51 mm to 6.02 ± 0.84 mm. The SAA of white AnGS were slightly lower than those of red AnGS with similarly-size granules but significantly higher than AnGS with smaller red granules. Compared with red AnGS, the extracellular polymeric substances of white AnGS were significantly reduced, mainly due to the higher intracellular iron content, resulting in lower heme c concentration. Thus, heme c may prove not to be an evaluative tool for measuring Anammox activity. Red and white AnGS, whether through self-aggregation or adsorption by hydroxyl apatite and other carriers, will face the fate of internal voids during particle size growth. White AnGS exhibited a more complex microbial community than red AnGS. Candidatus Brocadia was abundant in red AnGS and the abundance increased with increasing granule size. Candidatus Kuenenia and Candidatus Jettenia made significant contributions to denitrification in white AnGS. This study provides a new perspective on particle selection for anammox engineering applications.

BI Zhen; FU Hao; WANG Xue-ling; YUAN Yi-na; HUANG Yong
Zhongguo Huanjing Kexue/China Environmental Science, 2024 44 (11) - EI

摘要 : In this study, the effects of complex carbon sources on the performance of phosphorus enrichment and characteristics of microbial community were investigated in an anaerobic/aerobic alternatively operational biofilm reactor by using synthetic sewage wastewater containing VFAs, glucose and amino acid. The results showed that the utilization efficiency of complex carbon source reached 72.5% by adjusting the parameters of anaerobic hydraulic retention time, aerobic pH and DO concentration. The removal efficiency of total phosphorus and total nitrogen were 99.8% and 76.5%, respectively, with the concentration of phosphorus enrichment solution exceeding 50mg/L. The utilization complex carbon source changed the energy metabolism pathway of biofilm. Specifically, amino acids may participate in phosphorus metabolism as intracellular energy storage substances to compensate for the energy deficiency caused by PHA and glycogen, thus maintaining a good phosphorus removal and enrichment performance of the biofilm system. Meanwhile, the content of intracellular organophosphorus (OP) fluctuated with the activity of phosphorus uptake and release, whereas the content of inorganic poly-phosphorus (IP) changed a little. This phenomenon indicated that the phosphorus metabolism transferred from the IP pathway to the OP pathway. The abundance of hydrolytic bacteria and fermentative bacterium in biofilm increased significantly, which could provide small molecular organic carbon for phosphorus-accumulating bacteria (PAOs) metabolism, thus contribute the satisfactory performance of phosphorus removal and enrichment. The conclusion of present study exhibited the potential of biofilm system to simultaneously realize the phosphorus enrichment and total nitrogen removal via using complex carbon sources in sewage wastewater.

Min Ni; Yang Pan; Jiahui Gong; Zhiqiang Chen; Dapeng Li; Yong Huang
Bioresource Technology, 2024 -1 - EI SCIE

摘要 : A high phosphate (P) recovery concentration was achieved in pilot-scale biofilm sequencing batch reactor (BSBR) with a low carbon source (C) cost. Especially, a high-abundance glycogen-accumulating organisms (GAOs) (13.93–31.72%) was detected that was accompanied by a high P recovery concentration of BSBR. High-abundance GAOs obtain additional C through various C compensation pathways (split tricarboxylic acid cycle (TCA cycle), glyoxylate shunt and gluconeogenesis), thus reducing the need to compete with polyphosphate-accumulating organisms (PAOs) for C and weakening the adverse effects on P recovery by PAO cells. Under the action of N -acyl homoserine lactones (AHLs)-mediated quorum sensing (QS), GAOs promoted the secretion of a large amount of extracellular polymeric substances (EPS), which helped to realize the P recovery of EPS-dominated biofilms (68.02%–96.89%). This study provides a low-carbon technology for the recovery of high concentration P from municipal wastewater, and improves the ecological theory of P recovery in collaboration with GAOs and PAOs.

LI Wei; LI Xiang; YUAN Yan; HUANG Yong; FENG Zhen; LI Peng-fei
Zhongguo Huanjing Kexue/China Environmental Science, 2024 44 (11) - EI

摘要 : In order to solve the problems of low energy recovery rate and high energy consumption of traditional nitrification and denitrification in the treatment of high organic carbon and high ammonia wastewater by the anaerobic internal circulation reactor (IC)-anaerobic aerobic process (AO). Based on partial nitrification/anaerobic ammonia oxidation (PN/A), and IC high-efficiency methanation, complete autotrophic nitrogen removal process (CANON) to treat this kind of wastewater was designed, and the operating characteristics of IC-CANON in the energy recovery and nitrogen removal process were discussed, and the differences between IC-CANON and traditional IC-AO energy recovery, nitrogen removal stability and energy consumption level were evaluated. The results showed that when the C/N of IC effluent was 0.5~1, the nitrogen removal rate (NRR) of the CANON process reached the highest of 0.24kg/(m3·d), the nitrogen removal efficiency (NRE) was (76.2±5)%, and the minimum nitrogen removal contribution rate of Anammox was more than 70%. When influent C/N was 1.9~4, the NRR of first AO reached the highest of 0.14kg/(m3·d), and the NRE was (89.6±0.9)%. When the nitrogen load rate (NLR) of influent fluctuated at 0.22~0.32kg/(m3·d), the minimum contribution rates of CANON process NRE and Anammox remained above 70% and 65%, respectively. When the influent C/N increased to 2.2, the activity of Anammox in CANON was inhibited, and the NRE decreased from 76% to 45.4%. CANON had a strong resistance to NLR impact, but a poor resistance to organic load impact. Under the fluctuations of C/N and NLR of 0.6~6.6kg/(m3·d) and 0.06~0.16kg/(m3·d), the NRE of the first AO remained at about 90%, compared with Canon, the AO process had well resistance to NLR and organic load impact at the same time. When the NRR of the CANON process reached more than 0.21kg/(m3·d), the energy consumption of nitrogen removal is 3.5~3.6kWh/kgN, which can save (77.8±1.8)% compared with the AO process under the same influent and effluent, and the IC energy recovery rate increased from 62.1% to 89.2%.

Xiaoya Wang; Jun Zhang; Lu Li; Ye Zhu; Yue Zhang; Min Ni
Bioresource Technology, 2024 412 - EI SCIE

摘要 : Phosphorus recovery from wastewater is an effective method to alleviate the shortage of phosphorus resources. The biofilm phosphorus recovery process can realize simultaneous removal and enrichment of phosphorus in wastewater. In this study, a sequencing batch biofilm reactor was constructed to study the rapid phosphorus release and slow phosphorus release stages in the phosphorus recovery cycle. The relationship between high biofilm phosphorus storage capacity (P biofilm ), phosphorus recovery solution concentration, phosphorus uptake-release behavior and carbon source consumption were explored. The increase in phosphorus recovery solution concentration promotes the elevation of P biofilm, which, conversely promotes phosphorus release in the next recovery cycle. In addition, the distinct phosphorus uptake-release characteristics of extracellular polymeric substances and cells were illustrated. This study provides a theoretical foundation to elevate the phosphorus recovery efficiency and reduce carbon source consumption in biofilm phosphorus recovery process.

DANG Peng-ze; TAN Xin-wei; LI Xiang; YUAN Yan; HUANG Yong; LI Peng-fei
Zhongguo Huanjing Kexue/China Environmental Science, 2024 44 (10) - EI

摘要 : Using actual membrane wastewater as an example, this study explored the dynamic responses of methanogenesis and microbial communities in high-concentration organic nitrogen wastewater under ammonia stress, clarifying the ammonia inhibition threshold, inhibition form, inhibition mechanisms, and response strategies. The results indicate that the primary form of ammonia inhibition in anaerobic digestion (AD) treatment of high-concentration organic nitrogen wastewater is free ammonia (FA), with an inhibition threshold of (145±10) mg/L and a half-maximal inhibitory concentration of (244±10) mg/L. During AD treatment of high-concentration organic nitrogen wastewater, the key methanogenic microorganisms include Methanosaeta (acetotrophic methanogens), Methanomassiliicoccus and Methanomethylovorans (methylotrophic methanogens). The activities of Methanosaeta and Methanomethylovorans decline at ammonia nitrogen levels above 300mg/L and 900mg/L, respectively. Simultaneously, the functional microbes related to hydrogen and carbon dioxide production are inhibited when FA exceeds 140mg/L. This leads to the accumulation of small organic molecules with more than one carbon atom in the system, ultimately resulting in reduced chemical oxygen demand removal efficiency and incomplete release of ammonia nitrogen under high free ammonia (FA) conditions. After severe ammonia inhibition, recovery of the system can be achieved by adjusting the pH, but not through microbial acclimatization.

Miao Shi; Xiang Li; Pengze Dang; Qian Xu; Tianyu Huang; Yan Yuan
Bioresource Technology, 2024 409 - EI SCIE

摘要 : Understanding the effect of O 2 on the accumulation characteristics of NO 2 − -N and S 0 in the sulfur autotrophic denitrification (DSSADN) system is crucial for enhancing the denitrification efficiency of partial nitrification-anammox using DSSADN. The results revealed that in an environment without O 2 entry, the NO 2 − -N accumulation efficiency (NiAE) and S 0 accumulation efficiency (S 0 AE) of the DSSADN system reached 89.40 % and 93.41 %, respectively. Once system entered O 2, ORP value kept increasing. When ORP increased to −59.9 mV (DO = 0.1 mg/L), soxB and nirK gene expression rose and as well NiAE and S 0 AE continuously decreased to 48.13 % and 29.35 %. When ORP was above 30.9 mV (DO >0.2 mg/L) but below 81.0 mV (DO<0.4 mg/L), narG gene expression reduced and the relatively high sqr gene expression allowed NiAE and S 0 AE remained at 45.08 % and 33.31 %. O 2 promoted the synergistic effect of Thiobacillus and Azoarcus without the proliferation of nitrite oxidizing bacteria.

Lu Li; Yi Bu; Wen Feng; Kengo Kubota; Yang Pan; Yong Huang
Bioresource Technology, 2024 406 - EI SCIE

摘要 : Biomethane recovery from paper waste (PW) was achieved by mesophilic co-digestion with food waste. The feeding material containing 0%, 20%, 40% and 50% of PW in total solids (TS) were investigated in the long-term continuous operation. The results showed that the biogas production, pH, alkalinity and biodegradation of volatile solids (79.8 ± 3.6%) were stable for PW contents no more than 50%. The PW = 50% condition was considered the critical limit for the reasons of pump clogging, sufficient alkalinity (2.0 ± 0.3 g-CaCO 3 /L) and depletion of ammonia. Prokaryotic diversity indices decreased with the increased PW contents. Great shifts were observed in the prokaryotic communities before and after the PW contents reaches 50% as TS (18.4% as total weights). Biomethane recovery yields were deceasing from 445 to 350 NL-CH 4 /kg-fed-volatile-solids. The PW contents as 40% as TS (13.1% as total weights) obtained the optimal performance among all the feeding conditions.